STUDIES ON THE CHARACTERIZATION AND STRUCTURE OF THE IMMOBILIZATION ANTIGENS OF PARAMECIUM AURELIA
- 1 July 1965
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 96 (1) , 17-23
- https://doi.org/10.1042/bj0960017
Abstract
Methods for the extraction and purification of the immobilization antigens of Paramecium aurelia have been developed. The immobilization antigen was shown to be a protein of molecular weight 250,000. The amino acid content of 2 allelic antigens and 1 controlled by a separate genetic locus was determined and many differences were observed. The properties of antigen denatured by reduction and alkylation were examined. The molecular weight fell to 80,000 or perhaps less. Peptide maps of allelic types were more similar than those of types controled by different genetic loci. It is suggested that the antigen consists of 2 identical half-molecules held together by disulphide bonds. These half-molecules may contain further sub-units.Keywords
This publication has 18 references indexed in Scilit:
- A MODIFIED NINHYDRIN REAGENT FOR THE PHOTOMETRIC DETERMINATION OF AMINO ACIDS AND RELATED COMPOUNDSPublished by Elsevier ,2021
- Hemerythrin: Molecular Weight and Dissociation into SubunitsBiochemistry, 1963
- Immunological Assay of some Immobilizing Antigens of Paramecium aurelia, Variety 1Journal of General Microbiology, 1963
- The Preparation and Enzymatic Hydrolysis of Reduced and S-Carboxymethylated ProteinsJournal of Biological Chemistry, 1963
- A COMPARISON OF THE TRYPTIC PEPTIDES OBTAINED FROM IMMOBILIZATION ANTIGENS OF PARAMECIUM AURELIAProceedings of the National Academy of Sciences, 1962
- Internal standards for amino acid analysesBiochimica et Biophysica Acta, 1962
- Peptide Separation by Two-dimensional Chromatography and ElectrophoresisJournal of Biological Chemistry, 1959
- STUDIES ON THE IMMOBILIZATION ANTIGENS OF PARAMECIUM .2. ISOLATION1959
- STUDIES ON THE IMMOBILIZATION ANTIGENS OF PARAMECIUM .3. PROPERTIES1959
- DIET AND THE ERGOTHIONEINE CONTENT OF BLOODJournal of Biological Chemistry, 1953