Cloning a putative self-incompatibility gene from the pollen of the grass Phalaris coerulescens.
- 1 December 1994
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 6 (12) , 1923-1932
- https://doi.org/10.1105/tpc.6.12.1923
Abstract
In Phalaris coerulescens, gametophytic self-incompatibility is controlled by two unlinked genes: S and Z. A probable S gene has now been isolated and sequenced. This represents a novel self-incompatibility gene isolated from pollen in the multilocus system of a monocotyledonous plant. The gene is approximately 3 kb long, split by five introns, and exclusively expressed in the mature pollen. The deduced amino acid sequences from the S1, S2, and part of the S4 alleles showed that the protein has a variable N terminus and a conserved C terminus. The sequence of a complete mutant at the S locus indicated that mutations in the conserved C terminus, a thioredoxin-like region, led to loss of function. We propose that the gene has two distinct sections, a variable N terminus determining allele specificity and a conserved C terminus with the catalytic function. The gene structure and its deduced protein sequences strongly suggest that this monocotyledon has developed a self-incompatibility system entirely different from those operating in the dicotyledons. The possible interactions between S and Z genes in both pollen and stigma are discussedKeywords
This publication has 20 references indexed in Scilit:
- The S-Ribonuclease Gene of Petunia hybrida Is Expressed in Nonstylar Tissue, Including Immature AnthersPlant Physiology, 1994
- Loss of a histidine residue at the active site of S-locus ribonuclease is associated with self-compatibility in Lycopersicon peruvianum.Proceedings of the National Academy of Sciences, 1994
- Plant Self-Incompatibility: The key to specificityCurrent Biology, 1994
- Cloning and expression of a distinctive class of self-incompatibility (S) gene from Papaver rhoeas L.Proceedings of the National Academy of Sciences, 1994
- Pollen[mdash]Stigma Signaling in the Sporophytic Self-Incompatibility Response.Plant Cell, 1993
- Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea.Proceedings of the National Academy of Sciences, 1991
- Nucleotide sequence of a cDNA encoding a tobacco thioredoxinPlant Molecular Biology, 1991
- Characterization of Ribonuclease Activity of Three S-Allele-Associated Proteins of Petunia inflataPlant Physiology, 1991
- Style self-incompatibility gene products of Nicotlana alata are ribonucleasesNature, 1989
- THIOREDOXINAnnual Review of Biochemistry, 1985