Chromosome Damage Induced by Restriction Endonucleases Recognizing Thymine-rich DNA Sequences in Electroporated CHO Cells
- 1 January 1992
- journal article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 61 (3) , 323-328
- https://doi.org/10.1080/09553009214551001
Abstract
The influence of BrdU substitution of DNA in Chinese hamster cells on the frequencies of chromosomal aberrations induced by three restriction endonucleases which recognize thymine-rich sequences in DNA has been studied. The restriction enzymes chosen were Eco RI (recognition site G/AATTC), Sca I (AGT/ACT), and Dra I (TTT/AAA). A restriction enzyme that does not have thymine in the recognition sequence, Hae III (GG/CC), was also tried. These enzymes were introduced into cells by electroporation after two cell cycles of BrdU substitution and the aberration yields compared with that observed in non-substituted cells. Our results seem to indicate that the BrdU-substituted chromatin becomes resistant to the chromosome-breaking activity of the restriction enzymes recognizing thymine-rich DNA sequences. These observations are compared with the patterns of cutting of isolated DNA as shown by agarose gel electrophoresis.Keywords
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