Isolation and characterization of hypeptin from Pseudomonas sp.
- 1 January 1989
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 42 (10) , 1460-1464
- https://doi.org/10.7164/antibiotics.42.1460
Abstract
An antibiotic, named hypeptin, was isolated from the culture broth of a strain of Pseudomonas sp. The antibiotic was extracted with butanol and purified by chromatography on a Sephadex LH-20 column. The antibiotic is basic in nature. The dihydrochloride is soluble in aqueous alcohols and positive to ninhydrin and SAKAGUCHI'S reaction. A molecular formula, C44H71N13O15•2HCl was indicated by microanalysis and secondary ion MS. The IR spectrum and acid hydrolysis indicated it to be a peptide antibiotic. The stereochemistries of the amino acids produced by hydrolysis were clarified by HPLC using a chiral column. The antibiotic is active in vitro against a wide variety of anaerobic bacteria and aerobic Gram-positive bacteria.This publication has 1 reference indexed in Scilit:
- Bergey's Manual of Systematic Bacteriology, Volume 1.: Edited by Noel R. Krieg. The Williams & Wilkins Co., Baltimore, 1984, 964 pp. $80.00.International Journal of Systematic and Evolutionary Microbiology, 1985