A New Ganglioside Showing Choleragenoid-Binding Activity in Mouse Spleen1

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Abstract
A new ganglioside showing choleragenoid-binding activity was purified from mouse spleen and characterized. From the results of sugar-composition analysis, enzymatic hydrolysis, a permethylation study, 1 H-NMR spectroscopy, and negative-ion fast atom bombardment mass spectrometry, the structure of the ganglioside was determined to be as follows: This ganglioside contains a terminal tetrasaccharide structure identical with that of II 3 NeuGcα-Gg 4 Cer (GM1(NeuGc)). By means of a TLC-immunobinding assay and an enzyme-linked immunosorbent assay, the ganglioside was demonstrated to have almost the same choleragenoid-binding activity as GM1. Another ganglioside, that migrated faster than the new choleragenoid-binding ganglioside, was also purified from the same source material and identified as IV 4 GalNAcβ, IV 3 NeuGcα-Gg 4 Cer (GalNAc-GM1b(NeuGc)). Since, in the previous study, we demonstrated the existence of IV 3 NeuGcα-Gg 4 Cer (GM1b(NeuGc)) in mouse spleen (Nakamura, K. et al. (1984) J. Biochem . 96, 949–957), the results of this study suggest that the new choleragenoid-binding ganglioside is synthesized from GM1b(NeuGc) through GalNAc-GM1b(NeuGc).