Receptor-mediated release of endothelium-derived relaxing factor and prostacyclin from bovine aortic endothelial cells is coupled.
- 1 April 1988
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (7) , 2334-2338
- https://doi.org/10.1073/pnas.85.7.2334
Abstract
Bovine aortic endothelial cells were grown on microcarrier beads and were perfused with Krebs-Ringer solution. Endothelium-derived relaxing factor (EDRF) was bioassayed on a cascade of four strips of rabbit aorta, and prostacyclin was analyzed by RIA of 6-oxo-prostaglandin F1 alpha. The endothelial cells released EDRF and prostacyclin when stimulated with bradykinin and its analogues, or with ADP, ATP, arachidonic acid, and phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3). The detection of EDRF was potentiated by superoxide dismutase, and the relaxation of rabbit aortic strips induced by EDRF was antagonized by methylene blue. The release of EDRF and prostacyclin was inhibited by phorbol myristate acetate, R59022 (a diacylglycerol kinase inhibitor), and gentamycin. We suggest that the release of EDRF and prostacyclin is coupled and the initial common step is activation of a phospholipase C.Keywords
This publication has 51 references indexed in Scilit:
- Effects of Nonsteroidal Phospholipase Inhibitors and Glucocorticoids on Endothelium-Dependent Relaxations of Rabbit Aorta Induced by Different AgentsJournal of Cardiovascular Pharmacology, 1987
- Characterization of vascular relaxant factor released from cultured endothelial cells.Hypertension, 1987
- Pathways of arachidonic acid liberation in thrombin and calcium ionophore A23187-stimulated human endothelial cells: Respective roles of phospholipids and triacylglycerol and evidence for diacylglycerol generation from phosphatidylcholineBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1987
- Macrophage Cytotoxicity: Role for L-Arginine Deiminase and Imino Nitrogen Oxidation to NitriteScience, 1987
- Endothelium dependent vascular relaxation by arginine containing polypeptidesBiochemical and Biophysical Research Communications, 1986
- Release and properties of endothelium‐derived relaxing factor (EDRF) from endothelial cells in cultureJournal of Cellular Physiology, 1985
- Inositol trisphosphate, a novel second messenger in cellular signal transductionNature, 1984
- Synergistic functions of phorbol ester and calcium in serotonin release from human plateletsBiochemical and Biophysical Research Communications, 1983
- Mechanism of bradykinin-stimulated prostacyclin synthesis in porcine aortic endothelial cellsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
- The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholineNature, 1980