Bile Acids. Lxx. Preparative Separation of Kryptogenin from Companion Sapogenins by High Performance Liquid Chromatography
- 1 November 1984
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 7 (13) , 2591-2603
- https://doi.org/10.1080/01483918408067027
Abstract
Commercial samples of kryptogenin or its acetate can be purified by preparative high-performance liquid chromatography [HPLC] on a PrepPAK-500/Silica cartridge. The free alcohol is separated from accompanying sapogenin with a mixture of chloroform:methanol (50:1), whereas the acetates are separated well with a mixture of methylene chloride:hexane (2:3). The companion sapogenins, diosgenin and yamogenin, 25R- and 25S- isomers, were separated by analytical HPLC with hexane-isopropanol (100:1) or as the acetates with hexane:isopropanol (250:1). Characterization of kryptogenin and yamogenin was completed with 1H-NMR, IR and mass spectrometry.This publication has 10 references indexed in Scilit:
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