Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes.

Publisher Website
Google Scholar
Abstract
Five Ty insertion mutations were isolated at the LYS2 locus of S. cerevisiae. Genetic and physical analyses show that 4 Ty insertions are in the 5'' noncoding region of LYS2 and 1 is within the structural gene. Three of these Ty elements have been cloned and characterized. The Ty mutations differ from each other in restriction pattern, phenotypic effects on LYS2, reversion frequency and the nature of reversion events. Spt2 and spt3 mutations, known to suppress Ty insertions and their solo .delta. derivatives at HIS4, can also suppress the Lys- phenotype of a solo .delta. derivative of another Ty insertion (Ty128) at LYS2. Spt mutations can suppress Ty and .delta. mutations at both HIS4 and LYS2, suggesting that they are general for their effects on Ty and .delta. elements.