Automated High-Performance Liquid Chromatographic Technique for Determining Diltiazem and its Three Main Metabolites in Serum

Publisher Website
Google Scholar
Abstract
A sensitive and automatic method for the analysis of diltiazem and its three main metabolites in acidified serum is described using solid-liquid extraction on disposable extraction cartridges coupled to injection via a loop-column in an HPLC System. This method avoids the in-vitro degradation of diltiazem and its main metabolite MA (N-monodemethyl diltiazem) into M1 (desacetyl diltiazem) and M2 (desacetyl-N-demethyl diltiazem) and eliminates the numerous manipulations involved in liquid-liquid extraction.