Nitrogen regeneration by the subtropical marine copepod Eucalanus pileatus

Abstract
Ammonium release rates by individual marine copepods ( Eucalanus pileatus ) were examined kinetically over successive 10-min intervals after the animals were exposed to three concentrations of the diatom Thalassiosira fluviatilis as food. Food concentrations spanned those expected in the natural environment. “Well-fed” (3 mm 3T. fluviatilis l −1 ) copepods released ammonium significantly (p 100% of their N ingestion rates over the previous 18–22 h. Long-term (4–6 h) kinetic excretion experiments with “well-fed” animals indicated that, on average, release rates decreased with time after food removal, but patterns varied among individual copepods. In addition to ammonium release, o-phthalaldehyde reactive amino acid nitrogen was occasionally released by E. pileatus in large “spurt events” lasting from 20 to 60 min.

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