Enhanced Expression of Platelet-Activating Factor Receptor on Human Eosinophils by lnterleukin-3, lnterleukin-5 and Granulocyte- Macrophage Colony-Stimulating Factor
- 1 January 1996
- journal article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 111 (1) , 63-65
- https://doi.org/10.1159/000237419
Abstract
The effects of interleukin-3 (IL-3), IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) on the surface expression of platelet-activating factor (PAF) receptor on eosinophils was examined using a PAF receptor binding assay. Specific binding of [3H]WEB 2086, a specific ligand for PAF receptor, to peripheral blood eosinophils obtained from healthy individuals was significantly increased when the cells were cultured with IL-3, IL-5, or GM-CSF for 12 h, compared with untreated cells. The PAF-induced increase in intracellular calcium ion concentration in eosinophils was markedly augmented by exposure to IL-5 for 12 h. These results suggest that these cytokines modulate the responsiveness of eosinophils to PAF through enhancement of PAF receptor expression on the cell surface.Keywords
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