Requirement of the Activation-Induced Deaminase ( AID ) Gene for Immunoglobulin Gene Conversion
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- 15 February 2002
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 295 (5558) , 1301-1306
- https://doi.org/10.1126/science.1067308
Abstract
Three phenotypically distinct processes—somatic hypermutation, gene conversion, and switch recombination—remodel the functionally rearranged immunoglobulin (Ig) loci in B cells. Somatic hypermutation and switch recombination have recently been shown to depend on the activation-induced deaminase (AID) gene product. Here, we show that the disruption of the AID gene in the chicken B cell line DT40 completely blocks Ig gene conversion and that this block can be complemented by reintroduction of theAID complementary DNA. This demonstrates that theAID master gene controls all B cell–specific modifications of vertebrate Ig genes.Keywords
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