Transgenic plants expressing two Bacillus thuringiensis toxins delay insect resistance evolution
- 9 November 2003
- journal article
- Published by Springer Nature in Nature Biotechnology
- Vol. 21 (12) , 1493-1497
- https://doi.org/10.1038/nbt907
Abstract
Preventing insect pests from developing resistance to Bacillus thuringiensis (Bt) toxins produced by transgenic crops is a major challenge for agriculture. Theoretical models suggest that plants containing two dissimilar Bt toxin genes ('pyramided' plants) have the potential to delay resistance more effectively than single-toxin plants used sequentially or in mosaics. To test these predictions, we developed a unique model system consisting of Bt transgenic broccoli plants and the diamondback moth, Plutella xylostella. We conducted a greenhouse study using an artificial population of diamondback moths carrying genes for resistance to the Bt toxins Cry1Ac and Cry1C at frequencies of about 0.10 and 0.20, respectively. After 24 generations of selection, resistance to pyramided two-gene plants was significantly delayed as compared with resistance to single-gene plants deployed in mosaics, and to Cry1Ac toxin when it was the first used in a sequence. These results have important implications for the development and regulation of transgenic insecticidal plants.Keywords
This publication has 21 references indexed in Scilit:
- Long-term regional suppression of pink bollworm byBacillus thuringiensiscottonProceedings of the National Academy of Sciences, 2003
- Broccoli plants with pyramided cry1Ac and cry1C Bt genes control diamondback moths resistant to Cry1A and Cry1C proteinsTheoretical and Applied Genetics, 2002
- Control of Resistant Pink Bollworm ( Pectinophora gossypiella ) by Transgenic Cotton That Produces Bacillus thuringiensis Toxin Cry2AbApplied and Environmental Microbiology, 2002
- Economic, Ecological, Food Safety, and Social Consequences of the Deployment of Bt Transgenic PlantsAnnual Review of Entomology, 2002
- Development and Characterization of Diamondback Moth Resistance to Transgenic Broccoli Expressing High Levels of Cry1CApplied and Environmental Microbiology, 2000
- Two–toxin strategies for management of insecticidal transgenic crops: can pyramiding succeed where pesticide mixtures have not?Philosophical Transactions Of The Royal Society B-Biological Sciences, 1998
- Sustainability of Transgenic Insecticidal Cultivars: Integrating Pest Genetics and EcologyAnnual Review of Entomology, 1998
- Assessing the odds: The emergence of resistance to Bt transgenic plantsNature Biotechnology, 1997
- One gene in diamondback moth confers resistance to fourBacillus thuringiensis toxinsProceedings of the National Academy of Sciences, 1997
- Transgenic broccoli expressing aBacillus thuringiensis insecticidal crystal protein: Implications for pest resistance management strategiesMolecular Breeding, 1995