Superiority of Alcoholic Over Aqueous Fixation in the Histochemical Detection of Calcium

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Abstract
In three different cardiopathies produced experimentally in rats and known to be accompanied by calcifications in the myocardium or the coronary vessels, it has been demonstrated that, if a fixative is used, it must contain at least 80% alcohol for the early detection of calcium salt deposits. The use of neutral formalin fixation seems to be entirely unsuitable, since a positive reaction is obtained only in cases of macroscopically visible, massive deposits. For staining purposes after alcohol fixation, the alizarin or, even better, the von Kóssa method is quite successful.

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