IMMUNOHISTOLOGICAL STUDY OF EQUINE RENAL METALLOTHIONEIN

Publisher Website
Google Scholar
Abstract
The cellular site of metallothionein, a Cd- and Zn-binding protein, was investigated by immunofluorescent antibody technique in several horse, human, monkey, pig, bovine, mouse and rat organs. The antisera to the metallothionein was raised in rabbits. A single precipitin band was observed between antisera and antigen using both agar diffusion method and immunoelectrophoresis. The material reacting with anti-equine metallothionein antisera was detected in the renal tissue of horse, human and monkey, but not of pig, bovine, mouse and rat. Metallothionein was found mainly in the cytoplasms of proximal convoluted tubules and Henle loop of these kidneys. No remarkable positive fluorescence was found in other organs. The concentration of Cd in the kidneys of these animals was measured as 30-130 ppm in horse and monkey and 0.1-0.3 ppm in pig, cow, mouse and rat.

This publication has 0 references indexed in Scilit: