The distribution of peptidases in subcellular fractions from the mucosa of the small intestine of the rat
- 1 July 1963
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 88 (1) , 162-168
- https://doi.org/10.1042/bj0880162
Abstract
A method for the subcellular fractionation of intestinal mucosa is described. The subcellular fractions were characterized by electron microscopy and by determining the distributions of cytochrome oxidase and alkaline phosphatase in the subcellular fractions. The subcellular distribution of peptidases hydrolysing L-leucylglycine and glyclyglycine has been determined in intestinal mucosa. Most of the peptidase activity was located in the supernatant fraction. The remaining activity was found mainly in the microsomal fraction, with some in the mitochon-drial fraction.Keywords
This publication has 31 references indexed in Scilit:
- The digestive function of the epithelium of the small intestineBiochimica et Biophysica Acta, 1961
- A Study of Aminopeptidase Activity in the Stroma of Neoplastic Tissue, With a Comparison of Histochemical Techniques2JNCI Journal of the National Cancer Institute, 1959
- Lecithinase and lysolecithinase of intestinal mucosaBiochemical Journal, 1959
- The colorimetric determination of leucine aminopeptidase in urine and serum of normal subjects and patients with cancer and other diseasesCancer, 1958
- LABELING OF HISTONES AND OTHER NUCLEAR PROTEINS WITH L-LYSINE-U-C14 IN TISSUES OF TUMOR-BEARING RATS1958
- THE HISTOCHEMICAL DEMONSTRATION OF LEUCINE AMINOPEPTIDASEJournal of Histochemistry & Cytochemistry, 1957
- The purification of alkaline phosphatases of animal tissuesBiochemical Journal, 1954
- PEPTIDASES IN HUMAN BLOOD .2. THE HYDROLYSIS OF ADDITIONAL PEPTIDES BY NORMAL SERUM1954
- CYTOCHEMICAL STUDIES .5. ON THE ISOLATION AND BIOCHEMICAL PROPERTIES OF LIVER CELL NUCLEI1952
- A MICROSPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF CYTOCHROME OXIDASEJournal of Biological Chemistry, 1951