Use of an automatic DNA sequencer for S1 mapping: transcriptional analysis of the Streptomyces coelicolor A3(2) dnaK operon
Open Access
- 15 April 1997
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 149 (2) , 189-194
- https://doi.org/10.1016/s0378-1097(97)00074-8
Abstract
The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The −35 and −10 hexamers correspond to a σ70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from the CIRCE sequence characteristic of the Gram-positive heat-shock promoters.Keywords
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