TheBradyrhizobium japonicum phoBgene is required for phosphate-limited growth but not for symbiotic nitrogen fixation
Open Access
- 1 April 1998
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 161 (1) , 47-52
- https://doi.org/10.1111/j.1574-6968.1998.tb12927.x
Abstract
We identified by cloning and DNA sequence analysis the phosphate regulatory gene phoB of Bradyrhizobium japonicum. The deduced gene product displayed pronounced similarity to the PhoB protein of Sinorhizobium meliloti (71.4% identical amino acids), Escherichia coli (50.2%) and other bacterial species. Insertion of a kanamycin resistance cassette into phoB led to impaired growth of the B. japonicum mutant in media containing approximately 25 μM phosphate or less. A standard plant infection test using wild-type and phoB-defective B. japonicum strains showed that the phoB mutation had no effect on the symbiotic properties of B. japonicum with its soybean host plant.Keywords
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