Two-level factorial screening for influence of temperature, pH, and aeration on production of Serratia marcescens nuclease
- 1 October 1987
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 53 (10) , 2593-2596
- https://doi.org/10.1128/aem.53.10.2593-2596.1987
Abstract
Two high-nuclease-yielding mutants of Serratia marcescens, derived by chemical mutagenesis (W280, W355), and two strains with the pBR322 plasmid 403-SD2, carrying a nuclease gene and a chloramphenicol resistance gene [Escherichia coli CSH50(403-SD2) and S. marcescens CH30(403-SD2)] were investigated for nuclease production in a factorial shake flask experiment, with temperature (30 and 37 degrees C), pH (with or without CaCO3 tablets), and aeration (with or without baffles) as variable conditions. Yields varied 10-fold depending on the conditions investigated.This publication has 14 references indexed in Scilit:
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