Gene expression profiling in single cells within tissue
- 23 August 2005
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 2 (9) , 663-665
- https://doi.org/10.1038/nmeth786
Abstract
We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.Keywords
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