Lysosomal Hydrolases in Acanthamoeba sp.

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Abstract
SYNOPSIS. Homogenates of axenic Acanthamoeba sp. had ribonuclease, phosphatase, proteinase, α‐glucosidase, β‐N‐acetylglucosaminidase, and β‐glucuronidase activities; all had acid pH optima. After isopycnic density‐gradient centrifugation all showed the same relatively broad and unimodal distribution pattern, with a peak at a density of 1.17. This differs from the distribution of mitochondrial malate dehydrogenase and of peroxisomal urate oxidase and catalase. The findings are believed to reflect the occurrence of lysosomes in Acanthamoeba.