Methylation of replicating and post-replicated mouse L-cell DNA.
- 1 August 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (16) , 4919-4921
- https://doi.org/10.1073/pnas.80.16.4919
Abstract
[.alpha.-32P]dGTP into permeabilized cells and measured the degree of methylation at CpG sites by nearest-neighbor analysis. This method reveals a lag of .apprx. 1 min between DNA synthesis and the modification event. When methylation is inhibited by the addition of S-adenosyl-L-homocysteine in the presence of continued DNA synthesis, the resulting hemimethylated sites are methylated immediately after the release of inhibition. Evidently, the methylase activity in the cell allows immediate methylation, but conditions at the replication fork bring about a short delay in the onset of the modification reaction.This publication has 18 references indexed in Scilit:
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