Abstract
The vaccine (Blacksburg) strain of NDV harvested from allantoic fluid of chick embryos, after freezing at -70[degree]C and subsequent thawing, agglutinated and hemolyzed human 0-type red blood cells. The same virus, untreated, did not agglutinate or lyse fresh human red cells. However, if the red cells were stored at 4[degree]C for 5-7 days, they were agglutinated but not hemolyzed by the untreated virus.