Identification and quantitation of adenosine-3?:5?-cyclic monophosphate in plants using gas chromatography-mass spectrometry and high-performance liquid chromatography
- 1 July 1981
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 152 (3) , 195-201
- https://doi.org/10.1007/bf00385144
Abstract
Direct evidence has been obtained for the presence of adenosine-3′:5′-cyclic monophosphate (cAMP) in tobacco (Nicotiana tabacum L.) callus tissue cultures, bean (Phaseolus vulgaris L.) seedlings and immature kernels of sweet corn (Zea mays L.) through the use of a highly specific and sensitive gas chromatography-mass spectrometric assay. Levels of endogenous cAMP ranged from 70 to 126 pmol/g fresh weight. Corresponding levels of cAMP determined for the same samples using radioimmunoassay were consistently three to four times higher. Contrary to previous reports for citrus plants, measurable levels of cAMP could not be detected in young lemon leaves within the limits of detection of the mass-spectrometric assay method. In the case of tobacco callus tissue, the coumarin glucoside, scopolin, which was present in large amounts and showed similar chromatographic behaviour to cAMP, interferred strongly with the mass-spectrometric measurements of cAMP in inadequately purified extracts. The use of high-performance liquid chromatography, in addition to standard chromatographic purification methods, produced highly purified plant extracts for quantitation of cAMP and also provided a method for the separation of cAMP from its 2′:3′-isomer.Keywords
This publication has 19 references indexed in Scilit:
- The quantitation of adenosine 3′:5′‐cyclic monophosphate in cultured tobacco tissue by mass spectrometryFEBS Letters, 1981
- Adenosine 3′:5′-cyclic monophosphate, adenylate cyclase and a cyclic AMP binding-protein in Phaseolus vulgarisPhytochemistry, 1979
- Cyclic Adenosine 3′:5′-Monophosphate in Axenic Rye Grass Endosperm Cell CulturesPlant Physiology, 1978
- The Current Status of Cyclic Amp in Higher PlantsAnnual Review of Plant Physiology, 1977
- Attempts to Detect Cyclic Adenosine 3′:5′-Monophosphate in Higher Plants by Three Assay MethodsPlant Physiology, 1976
- Plant NucleasesAnnual Review of Plant Physiology, 1975
- Adenosine 3′, 5′-cyclic monophosphate in higher plants: Assay, distribution and age-dependencyBiochimica et Biophysica Acta (BBA) - General Subjects, 1974
- Distribution of cyclic AMP in maize seedlingsPlant Science Letters, 1973
- Intracellular Levels of Cyclic Nucleotides during Cell Enlargement and Cell Division in Excised Tobacco Pith TissuesDifferentiation, 1973
- Mass spectrometry of nucleic acid components. Trimethylsilyl derivatives of nucleotidesJournal of the American Chemical Society, 1971