Frequencies of Fetal Nucleated Red Blood Cells in Maternal Blood during Different Stages of Gestation
- 1 December 1998
- journal article
- Published by S. Karger AG in Fetal Diagnosis and Therapy
- Vol. 13 (6) , 375-379
- https://doi.org/10.1159/000020873
Abstract
Nucleated red blood cells (NRBCs) isolated by a triple density gradient from 100 ml peripheral blood samples of 100 pregnant women and 30 women postpartum were subjected to morphological analysis and PCR quantitation. The number of NRBCs steadily increased from 5.3 (frequency: 2.4 × 10–7) in early gestation to 98.2 (frequency: 4.2 × 10–6) near term. The number of male cells increased from 6 (frequency: 2.7 × 10–7) in early gestation to a peak of 31 (frequency: 1.48 × 10–6) in the second trimester, and slightly decreased to 27 (frequency: 1.31 × 10–6) near term. Both NRBCs and male fetal cells rapidly disappeared after delivery. The result implies that a significant proportion of NRBCs in maternal blood are of fetal origin before 24 weeks of gestation while in late gestation the majority of NRBCs may be of maternal origin.Keywords
This publication has 5 references indexed in Scilit:
- The Proportion of Fetal Nucleated Red Blood Cells in Maternal Blood: Stimation by FACS AnalysisPrenatal Diagnosis, 1997
- Male fetal progenitor cells persist in maternal blood for as long as 27 years postpartum.Proceedings of the National Academy of Sciences, 1996
- Isolating fetal cells from maternal blood. Advances in prenatal diagnosis through molecular technologyPublished by American Medical Association (AMA) ,1993
- One-step enrichment of nucleated red blood cellsJournal of Immunological Methods, 1993
- First trimester prenatal diagnosis of trisomy 21 in fetal cells from maternal bloodThe Lancet, 1992