Effective removal of albumin from serum
- 10 October 2005
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 5 (15) , 3831-3835
- https://doi.org/10.1002/pmic.200401235
Abstract
The protein constituents of serum can range from grams to picograms per liter, making it technically difficult to achieve in‐depth proteomic analysis. Removal of highly abundant proteins, such as albumin, coupled to powerful protein separation methods is required for increased sample load, thus facilitating detection and identification of low‐abundant proteins. We report here a chemical‐based extraction method for the effective and specific removal of albumin from serum.Keywords
This publication has 11 references indexed in Scilit:
- The Human Plasma ProteomeMolecular & Cellular Proteomics, 2002
- Detection of Cardiac Troponin I Early after Onset of Chest Pain in Six PatientsClinical Chemistry, 2002
- Proteomic analysis of human plasma: Failure of centrifugal ultrafiltration to remove albumin and other high molecular weight proteinsProteomics, 2001
- New zwitterionic detergents improve the analysis of membrane proteins by two‐dimensional electrophoresisElectrophoresis, 1998
- Improvement of the solubilization of proteins in two‐dimensional electrophoresis with immobilized pH gradientsElectrophoresis, 1997
- Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide GelsAnalytical Chemistry, 1996
- A general method for fractionation of plasma proteins. Dye-ligand affinity chromatography on immobilized Cibacron blue F3-GABiochemical Journal, 1982
- A Simple Method for the Preparation of Human Serum AlbuminVox Sanguinis, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Two new staining procedures for quantitative estimation of proteins on electrophoretic stripsBiochimica et Biophysica Acta, 1963