Inhibition of cytokinesis in the microspores of Tradescantia bracteata Small, by caffeine

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Abstract
In isolated stamens of Tradescantia bracteata planted in nutrient medium cytokinesis in microspores was inhibited by caffeine and binucleate pollen grains were formed. Both the nuclei were of similar shape, size and structure and did not undergo further changes. It seems that separation of the polarly differentiated microspore protoplast by a cell wall is a necessary condition for differentiation of the gametophyte nuclei to generative and vegetative.

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