Tritiated Water Vapour Exchange Method for the Evaluation of Whole Plant Diffusion Resistance

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Abstract
Water vapour exchange between plant and atmosphere was studied by using THO as a tracer. Pulses of THO vapour were briefly injected into the airflow of an open circuit plant chamber. Water vapour exchange was calculated from the difference in total quantity of THO escaping from the plant chamber with and without a plant The amount of THO exchanged was closely dependent on the product of total leaf area × mean leaf conductance. The method is suitable for measuring on the same plant short term variations in leaf conductance following changes in environmental conditions.