A simple apparatus for generating stretched polyacrylamide gels, yielding uniform alignment of proteins and detergent micelles*
- 1 January 2001
- journal article
- Published by Springer Nature in Journal of Biomolecular NMR
- Vol. 21 (4) , 377-382
- https://doi.org/10.1023/a:1013336502594
Abstract
Compressed and stretched polyacrylamide hydrogels previously have been shown to offer a robust method for aligning proteins. A simple, funnel-like apparatus is described for generating uniformly...Keywords
This publication has 13 references indexed in Scilit:
- Persistence of Native-Like Topology in a Denatured Protein in 8 M UreaScience, 2001
- What is the average conformation of bacteriophage T4 lysozyme in solution? a domain orientation study using dipolar couplings measured by solution NMRJournal of Molecular Biology, 2001
- A set of HNCO-based experiments for measurement of residual dipolar couplings in 15N, 13C, (2H)-labeled proteinsJournal of Biomolecular NMR, 2000
- Solution NMR of proteins within polyacrylamide gels: Diffusional properties and residual alignment by mechanical stress or embedding of oriented purple membranesJournal of Biomolecular NMR, 2000
- Tunable alignment of macromolecules by filamentous phage yields dipolar coupling interactionsNature Structural & Molecular Biology, 1998
- Measurement ofJand Dipolar Couplings from Simplified Two-Dimensional NMR SpectraJournal of Magnetic Resonance, 1998
- Characterization of magnetically oriented phospholipid micelles for measurement of dipolar couplings in macromoleculesJournal of Biomolecular NMR, 1998
- Use of dipolar 1H–15N and 1H–13C couplings in the structure determination of magnetically oriented macromolecules in solutionNature Structural & Molecular Biology, 1997
- High-resolution heteronuclear NMR of human ubiquitin in an aqueous liquid crystalline medium.Journal of Biomolecular NMR, 1997
- Nuclear magnetic dipole interactions in field-oriented proteins: information for structure determination in solution.Proceedings of the National Academy of Sciences, 1995