7Li Nuclear‐Magnetic‐Resonance Study of Lithium Binding to Myo‐Inositol Monophosphatase
- 1 August 1996
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 240 (1) , 288-291
- https://doi.org/10.1111/j.1432-1033.1996.0288h.x
Abstract
The interaction of Li+ with myo‐inositol monophosphatase was studied by 7Li‐NMR spectroscopy. Li+ binding to the enzyme induces a downfield shift and broadening of the 7Li‐NMR signal. Changes of the chemical shift were used to follow the titration of the enzyme with lithium and to determine a dissociation constant, Kd= (1.0 ± 0.1) mM. Only one major binding site/enzyme subunit was inferred. The complex forms independently of the presence of inorganic phosphate. Metals from the group IIa of the periodic table compete with Li+ binding with the affinity increasing in the order Mg2+ < Ca2+ Be2+. In contrast to lithium, their binding is enhanced by phosphate.Keywords
This publication has 25 references indexed in Scilit:
- Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genesPublished by Elsevier ,2004
- 3-Dimensional interactions between inositol monophosphatase and its substrates, inhibitors and metal ion cofactorsJournal of the Chemical Society, Perkin Transactions 1, 1995
- Structural Studies of Metal Binding by Inositol Monophosphatase: Evidence for Two-Metal Ion CatalysisBiochemistry, 1994
- Structural Analysis of Inositol Monophosphatase Complexes with SubstratesBiochemistry, 1994
- The binding of substrates and inhibitors to the metal center of myoinositol monophosphataseFEBS Letters, 1994
- Bovine inositol monophosphataseFEBS Letters, 1993
- Structural characterization of myo-inositol monophosphatase from bovine brain by secondary structure prediction, fluorescence, circular dichroism and Raman spectroscopyBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1993
- Competition between Li+ and Mg2+ for ATP and ADP in aqueous solution: A multinuclear NMR studyJournal of Inorganic Biochemistry, 1991
- Kinetic studies with myo-inositol monophosphatase from bovine brainBiochemistry, 1990
- Multinuclear magnetic resonance studies of metal ion binding sites of phosphoglucomutaseBiochemistry, 1985