Growth of Japanese B Encephalitic Virus in the Yolk of the Developing Egg
- 1 June 1946
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 62 (2) , 105-108
- https://doi.org/10.3181/00379727-62-15387
Abstract
Expts. to increase the strength of the Japanese B encephalitic virus (Nakayama strain) in the hen''s egg indicated that the highest potency would be obtained in chick embryos by using 7- or 8-day-old embryonated eggs inoculated into the yolk with 0.5-ml. amts. of mouse brain virus of a potency of 10-8.5 or better, and incubating 36 hours at 95[degree] to 96[degree]F. Because of the constant lethal effect after 48 hrs., the inoculation of the egg yolk is of value for titrations of this virus and for use in other quantitative expts.Keywords
This publication has 1 reference indexed in Scilit:
- A Vaccine Against Japanese B Encephalitis Prepared from Infected Chick EmbryosExperimental Biology and Medicine, 1946