Subcellular Localization of “Peripheral‐Type” Binding Sites for Benzodiazepines in Rat Brain

Publisher Website
Google Scholar
Abstract
The binding of [3H]Ro 5–4864, a specific ligand for “peripheral-type” benzodiazepine binding sites and [3H]Ro 15–1788, a specific ligand for the central benzodiazepine receptors, was determined in subcellular fractions of rat brain. As previously reported, the highest levels of “peripheral-type” benzodiazepine binding sites and benzodiazepine receptors were found in the crude P1, and P2 fractions, respectively. Purification of these crude fractions revealed that high levels of both [3H]Ro 5–4864 and [3H]Ro 15–1788 binding were present in the mitochondrial and synaptosomal fractions. In contrast, the purified nuclei and myelin contained low levels of both [3H]Ro 5–4864 and [3H]Ro 15–1788 binding.