Role of modified nucleosides in tRNA: Effect of modification of the 2-thiouridine derivative located at the 5'-end of the anticodon of yeast transfer RNA2Lys
- 1 March 1976
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (3) , 523-536
- https://doi.org/10.1093/nar/3.3.523
Abstract
Yeast tRNA Lys2 codes preferentially for AAA and contains a 2-thiouridine derivative (U) at the 5'-position of the anticodon. Removal of the 2-thio group from U by treatment with CNBr did not affect the amino acid accepting activity of the modified tRNA Lys2. CNBr treated tRNA Lys2 was active in protein synthesis but with a much reduced efficiency. Although the modified tRNA Lys2 was recognized by elongation factor (EF) T, the EFT dependent binding to ribosomes to tRNA Lys2 (CNBr) was markedly decreased.Keywords
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