Optimizing Nephelometric Measurement of Specific Serum Proteins: Evaluation of Three Diluents
- 1 December 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 20 (12) , 1548-1552
- https://doi.org/10.1093/clinchem/20.12.1548
Abstract
Three diluents were studied, to determine which is the best for the automated immunochemical measurement of specific serum proteins. Nine serum proteins (orosomucoid, α1-antitrypsin, α2-macroglobulin, haptoglobin, transferrin, C3, IgG, IgA, and IgM) were measured in physiological saline (9 g NaCI/liter), tris(hydroxymethyl)aminomethane buffer (0.01 mol/liter; pH 7.4), and physiological saline containing polyethylene glycol ("PEG 6000," 40 g/liter). Criteria were: reaction rate, analysis rate, carryover between samples, steady-state approximation, precision, and correlation with other methods. Saline containing polyethylene giycol is the best of the three diluents for use in continuous-flow nephelometric analysis of serum proteins.Keywords
This publication has 0 references indexed in Scilit: