Discrimination between M2 and M4 antimitochondrial antibodies in primary biliary cirrhosis
- 1 December 1986
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 6 (12) , 1049-1056
- https://doi.org/10.1007/bf01141026
Abstract
10 sera were studied from patients with primary biliary cirrhosis (PBC), that were anomalous in their reactivity against mitochondrial antigens as detected by Western blotting. They had low reactivity against the major, M2 reactive antigen (Mr for beef heart mitochondria, 74 Kd) but reacted against an antigen of Mr 52 Kd (species independent) which was apparently inaccessible in submitochondrial particles (SMP) on ELISA and which was not present in chloroform-released ATPase preparations. In all respects this differed from the characteristics of the M2 antigens and it is concluded that these sera are detecting predominantly the M4-reactive antigen.This publication has 26 references indexed in Scilit:
- Reaction pattern of mitochondrial antibodies of primary biliary cirrhosis (PBC) is species specific but not organ specificJournal of Bioenergetics and Biomembranes, 1987
- Antimitochondrial antibodies in primary biliary cirrhosisJournal of Hepatology, 1986
- The PBC-specific antigenMolecular Aspects of Medicine, 1985
- Mitochondrial Antibodies in Primary Biliary Cirrhosis: Species and Nonspecies Specific Determinants of M2 AntigenHepatology, 1985
- ATPASE-ASSOCIATED ANTIGEN (M2): MARKER ANTIGEN FOR SEROLOGICAL DIAGNOSIS OF PRIMARY BILIARY CIRRHOSISThe Lancet, 1982
- SEROLOGICAL CLASSIFICATION OF CHRONIC CHOLESTATIC LIVER DISEASE BY THE USE OF TWO DIFFERENT TYPES OF ANTIMITOCHONDRIAL ANTIBODIESThe Lancet, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Maturation of the head of bacteriophage T4Journal of Molecular Biology, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970