Immunohistochemical localization of protein kinase C isozymes.

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Abstract
We report the immunohistochemical localization of protein kinase C isozymes (types I, II, III and ζ) in various organs using the monospecific monoclonal antibodies: MC-la, MC-2a and MC-3a, and a polyclonal antibody for a synthetic polypeptide of ζ, reviewing results reported previously (2-4, 20, 21, 23), and discussing the technical aspect of immunohistochemistry on protein kinase C. For light microscopy, tissues were fixed and cryo-sections or paraffin sections were made. They were stained by PAP, ABC and immunofluorescent technique. For immuno-electrom microscopy, pre-embedding immunoperoxidase and post-embedding immuno-gold technique were done. By light microscopic analysis, tissue specific differential localization of isozymes was observed. Three isozymes (types I, II and III) were consistently localized in the cytoplasm of various kinds of cell. On the contrary, ζ-related protein was located in the nucleus of Purkinje cells. The increased staining for protein kinase C isozymes was observed in human thyroid cancer tissues and cultured glioma cells. By immuno-electron microscopy, diffuse localization of protein kinase C (type III) in the cytoplasm of rod bipolar cells of the retina was observed. Type II isozyme was densely localized in the hyaluromere of blood platelets, and translocated to the cell membrane by TPA administration. The immunohistochemical techniques were very effective in elucidating the tissue specific localization of protein kinase C isozymes and their pathological changes.

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