The Metabolism of Lactate and Pyruvate by Pseudomonas AM1

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Abstract
Summary: Pseudomonas AMI, which can grow on C1 compounds, also grows well on pyruvate and lactate. Extracts of the pyruvate-grown organism catalysed the synthesis of only small amounts of phosphoeno/pyruvate directly from pyruvate. Experiments tracing the incorporation of radioactivity into bacterial constituents from pyruvate or lactate, each labelled in the C-1 or C-3 position, indicated that these substrates are mainly assimilated after removal of the C-1 atom. A mutant, C5, of Pseudomonas AMI was isolated by a procedure intended to select organisms lacking phosphoeflo/pyruvate carboxylase. Although this enzyme was present in mutant C5, growth on lactate or pyruvate was impaired and there was no growth on C1 compounds or ethanol. Good growth on each of these substrates was restored by a supplement of glyoxylate or glycollate. A scheme is proposed for the assimilation via malate synthase and phosphoenol-pyruvate carboxykinase of the C2 fragment produced from pyruvate and lactate.

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