Gel Filtration Profile of Circulating Immunoreactive Thyrotropin and Subunits of Myxedematous Sera

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Abstract
Five sera from hypothyroid patients were passed through gel-filtration columns and the collected fractions were analyzed in three different radioimmunoassays (hTSH-anti-hTSH; hTSHβ-anti-pTSH; hLH-α-anti hLHα). For each immunological system, cross reactions were carefully studied. In the hTSH system one main peak was observed at the elution position of the entire molecule, with variable contributions in the subunits area. In the α subunit system, every profile showed an asymmetric peak; corresponding roughly to the position of the entire molecule; only two sera exhibited small amounts of free α chain. In the β subunit system, a high molecular weight (100,000 or more) immunoreactive protein was present in every serum. Moreover, cross-reacting intact molecule was eluted as a second peak and in four sera free β chain was detected. The significance of the big β subunit, detectable only in the β assay, is not elucidated.

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