Gas-Liquid Chromatography of Methyl Esters of Fatty Acid from Human and Chicken Brain Lipids.

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Abstract
Separations were achieved by chromatography of methyl esters on a 10 foot diethylene glycol polyester succinate column at 240[degree] C. Chromatography of hydrogenated esters gave well-defined peaks of all even-numbered esters from C14 through C24 and odd-numbered esters from C17 through C23. Peaks corresponding to C16 and C18 branched chain esters were also observed. Further, but ill-defined peaks corresponding to C25 and C26 esters were obtained. Chromatography of untreated methyl esters gave 25 well-resolved peaks representing saturated and unsaturated normal fatty acids. Over half of these peaks could be identified and tentative identifications assigned to others. Even-numbered alpha hydroxy fatty acids from C18 through C24 were determined by increases in odd chain acids after oxidation of hydrogenated fatty acids and constitute 4-6.8% of total brain fatty acids.

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