USE OF PREFORMED CAVITIES IN RABBITS FOR THE QUANTITATION OF LEUKOCYTE CHEMOTAXIS CAUSED BY BACTERIAL LIPOPOLYSACCHARIDES

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Abstract
Wound chambers implanted s.c. in rabbits proved suitable for measurement of leukocyte chemotaxis. Injection of bacterial [Bacteroides fragilis ssp. fragilis and Veillonella parvula] lipopolysaccharide (LPS) in the chambers 6 days after implantation was followed by a marked increase of polymorphonuclear leukocytes in the wound chamber fluid, the number of which was dependent on the time after application of LPS. Up to a certain amount of LPS, the concentration of leukocytes in the chamber fluid was dose-dependent. The histopathological appearance of the granulation tissue lining the chamber wall 1 day after the injection of LPS from Veillonella revealed aggregation of blood cells plugging the lumina of small vessels and many eosinophilic leukocytes.

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