Purification and Serology of Peanut Mottle Virus

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Abstract
Peanut mottle virus (PMV) was purified from pea (Pisum sativum Little Marvel) by extraction in 0.1 M tris-HCl, pH 8.0, containing 0.05 M EDTA and 0.02 M Na2SO3, followed by clarification with 25% chloroform and precipitation with polyethylene glycol. Virus suspended in 0.01 M tris, pH 8.0, containing 0.5 M urea and 0.001 M EDTA sedimented primarily as a single component. Yields of 10-25 mg/kg of tissue were obtained. Both yield and infectivity were enhanced by Na2SO3 and EDTA. Specific antisera, with microprecipitin titers of 1/256, reacted with PMV-infected pea, soybean and peanut from the greenhouse or field in agar immunodiffusion tests containing sodium dodecyl sulfate (SDS). Purified PMV at 0.2 mg/ml was completely degraded at SDS concentrations > 0.05% in 0.005 M tris-HCl, pH 8.0. PMV was not related serologically to bean yellow mosaic, clover yellow vein or soybean mosaic viruses as shown by SDS gels or enzyme-linked immunosorbent assay.

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