Two "early" mRNA species in adenovirus type 2-transformed rat cells

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Abstract
MRNA isolated from adenovirus 2[Ad2]-infected [human cervical carcinoma] HeLa cells at early times during the productive cycle and from 2 lines of Ad2-transformed rat embryo cells (F17 and T2C4) was fractionated on sucrose gradients after disaggregation. Viral mRNA species were identified by hybridization across such gradients with the separated strands of [EcoRI and HpaI] restriction endonuclease fragments of 32P-labeled DNA known to be complementary to Ad2 early and Ad2-transformed cell mRNA. mRNA transcribed from the left-hand 14% of the Ad2 genome comprised 2 species, 16 to 17S and 20 to 21S: the same sized mRNA were present at early times during productive infection and in the 2 transformed rat cell lines. Direct comparison of the sequences present in these 2 mRNA species by additional saturation hybridizations suggests that they are not related to one another. Three additional regions of the Ad2 genome, all of which are located in the right-hand 40% of the Ad2 genome, are complementary to early mRNA sequences: each of these appears to specify one major mRNA species of about 22S. Thus, 5 major species of Ad2 early mRNA were identified. Two of these, copied from the left-hand 14% of the viral genome, are also present in Ad2-transformed rat cells.