Improvements in the microtitre GM1 ganglioside enzyme‐linked immunosorbent assay for Escherichia coli heat‐labile enterotoxin

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Abstract
A variant of the microtitre GM1-ELISA for Escherichia coli heat-labile enterotoxin was studied. The test was improved by both reducing the assay time from 21/2 d to 8 h and by determining the most appropriate GM1 coating concentration. Coating the plates with .gtoreq. 3 .mu.g of GM1/ml yielded a maximal sensitivity and ensured a linear relationship between the enterotoxin concentration and the extinction observed when using the final assay-procedure. Thus an optimal accuracy was obtained. This ELISA was 4- to 8-times more sensitive than the Vero cell monolayer assay. The sensitivity of this ELISA and of the chinese hamster ovary cell monolayer assay were identical.