Influence of some mononucleotides and their corresponding nucleosides on the metabolism of carbohydrates in the isolated rat diaphragm muscle
- 1 November 1965
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 97 (2) , 565-568
- https://doi.org/10.1042/bj0970565
Abstract
1. The influence of ATP on glucose metabolism was studied in the isolated rat diaphragm; it was shown that ATP increases the oxidation of glucose and the aerobic conversion of glucose into lactate, whereas it decreases glycogen synthesis. There was no influence of ATP on the anaerobic formation of lactate from glucose. 2. A maximum effect of ATP on the oxidation of glucose (about 160% increase) was obtained in the presence of 10mm-ATP; in the presence of 2mm-ATP the effect was about 65%, and was approximately constant from 10 to 90min. incubation period. 3. In a phosphate-free tris-buffered medium the oxidation of glucose was considerably decreased, but the percentage stimulation by ATP was about the same as in a phosphate-buffered medium. 4. ATP was shown to increase the oxidation of fructose, glucose 6-phosphate, glucose 1-phosphate, fructose 1,6-diphosphate and, to a much smaller extent, pyruvate. 5. ADP stimulated the oxidation of glucose to the same extent as ATP at a concentration of 2mm and the effect with AMP was only slightly less; IMP and adenosine had only a small stimulatory effect at this concentration, whereas inosine had no effect.This publication has 3 references indexed in Scilit:
- The metabolism of glucose 1-phosphate and glucose 6-phosphate and their influence on the metabolism of glucose in rat-diaphragm muscleBiochemical Journal, 1964
- Enzymic Conversion of Mononucleotides by Rat Diaphragm in vitro.Acta Physiologica Scandinavica, 1958
- The influence of insulin on carbohydrate metabolism in the isolated diaphragm muscle of normal and alloxan diabetic ratsProceedings of the Royal Society of London. B. Biological Sciences, 1955