Differential effects of gabaculin and laevulinic acid on protochlorophyllide regeneration

Abstract
The effects of gabaculin (3‐amino 2,3‐dihydrobenzoic acid) and laevulinic acid on the regeneration of protochlorophyllide from exogenous δ‐aminolaevulinic acid in leaves of dark‐grown barley (Hordeum vulgare) after a brief light treatment were compared. Gabaculin, a potent inhibitor of chlorophyll biosynthesis, did not inhibit this process showing that it affects the formation of δ‐aminolaevulinic acid rather than its further metabolism. Laevulinic acid, which is an inhibitor of δ‐aminolaevulinic acid dehydratase, prevented regeneration of protochlorophyllide provided pools of intermediates in the biosynthetic sequence were depleted. Formation of relatively large amounts of protochlorophyllide in some experiments suggests a lack of control in the utilization of δ‐aminolaevulinic acid for protochlorophyllide synthesis.

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