Divinyl ether synthase from garlic (Allium sativum L.) bulbs: sub‐cellular localization and substrate regio‐ and stereospecificity
Open Access
- 17 June 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 388 (2-3) , 112-114
- https://doi.org/10.1016/0014-5793(96)00536-4
Abstract
Sub‐cellular localization and some properties of 13‐hydroperoxide‐specific divinyl ether synthase from garlic bulbs were studied. Sub‐cellular fractions from garlic bulbs were incubated with [1−14C](9Z,11E,13S)‐13‐hydroperoxy‐9,11‐octa‐decadienoic acid (13‐HPOD). The predominant part of divinyl ether synthase activity from garlic bulbs was found in the microsomal fraction. The enzyme utilizes 13(S)‐HPOD as its preferential substrate. Other hydroperoxides, including 9(S)‐HPOD, gave much poorer yields of divinyl ethers. Unreacted hydroperoxide after incubation of 13(R,S)‐HPOD with enzyme was composed of up to 94% 13(R)‐HPOD. Thus, divinyl ether synthase possesses stereoselectivity, utilizing preferentially the (S)‐enantiomer.Keywords
This publication has 11 references indexed in Scilit:
- The lipoxygenase pathway in garlic (Allium sativum L.) bulbs: detection of the novel divinyl ether oxylipinsFEBS Letters, 1995
- Physiologically Active Products of Plant Lipoxygenase PathwayPublished by Springer Nature ,1995
- A gas-liquid chromatographic method for steric analysis of 2-hydroxy, 3-hydroxy, and 2,3-dihydroxy acidsChemistry and Physics of Lipids, 1994
- Recent investigations into the lipoxygenase pathway of plantsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1991
- An isotopic study (2H and 18O) of the enzymic conversion of linoleic acid into colneleic acid with carbon chain fracture: the origin of shorter chain aldehydesJournal of the Chemical Society, Perkin Transactions 1, 1991
- Total synthesis of colneleic acidThe Journal of Organic Chemistry, 1990
- Stereospecific removal of the pro-R hydrogen at C-8 of (9S)-hydroperoxyoctadecadienoic acid in the biosynthesis of colneleic acidJournal of the Chemical Society, Perkin Transactions 1, 1990
- Biomimetic total synthesis of colneleic acid and its function as a lipoxygenase inhibitorTetrahedron Letters, 1987
- LipoxygenasesPublished by Elsevier ,1980
- Novel divinyl ether fatty acids in extracts of Solanum tuberosumChemistry and Physics of Lipids, 1973