Studies on the Ascorbic Acid Synthesis in Animal Tissues with the Reconstructed Enzyme Systems***

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Abstract
The enzyme system consisting of L-gulonolactone dehydrogenase, TPN-L-hexonate dehydrogenase and lactonase-I was reconstructed, and the actual formation of L-ascorbic acid was observed from D-glucuronic acid and L-gulonic acid. Under the condition tested the absence of lactonase-I resulted in the failure to synthesize L-ascorbic acid, although the synthesis occurred from D-glucuronolactone or L-gulonolactone. The addition of barbital to the reconstructed system inhibited the L-ascorbic acid synthesis. Chloretone did not affect the system. The enzyme system consisting of TPN-L-hexonate dehydrogenase and DPN-L-gulonate dehydrogenase produced L-xylulose from D-glucuronic acid. With the addition of L-gulonolactone dehydrogenase and lactonase-I, human liver homogenates became capable of forming L-ascorbic acid from L-gulonic acid.

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