Abstract
To provide a framework for use of the doubly labelled water method to measure energy expenditure in order to validate dietary instruments for the assessment of energy. Review and description of the use of doubly labelled water method for use as a biomarker for habitual energy intake. The doubly labelled water method has a relative accuracy of 1% and within-subject precision of 5 to 8%. Comparison of self-reported energy intake with energy expenditure demonstrated that over one-third of individuals may underreport energy intake by more than 25%. The doubly labelled water method, although expensive and dependent on non-routine laboratory instrumentation, is an excellent biomarker of energy intake.