Cytotoxicity Test with Frozen Lymphocytes

Publisher Website
Google Scholar
Abstract
Technics for the preparation and freezing of highly purified lymphocyte suspensions are given. Cell recovery was about 75% and viability of recovered cells was as great as that of freshly prepared lymphocytes. No loss or alteration in antigenicity of frozen-thawed lymphocytes occurred as judged by cytotoxicity testing. The availability of frozen lymphocytes sufficiently viable to be employed in cytotoxicity testing should facilitate investigations of human transplantation antigenic systems,.

This publication has 1 reference indexed in Scilit: