Low-temperature requirements for ascospore germination and growth of Hypoxylon diathrauston

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Abstract
Ascospores of Hypoxylon diathrauston Rehm germinated only at freezing temperatures or required pretreatments at −3 °C to enable them to germinate at 12 °C. They required from 10 to 30 days to germinate in our experiments. They germinated by two, but occasionally one, three, and even four, germ tubes growing through pores in the relatively thick inner wall at each end of the slit after its enlargement. Cultures grew at 0 °C with an optimum at 12–15 °C, and a maximum between 18 and 21 °C.

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